School of Medicine

Research

Proteomics & Metabolomics Core 

The Proteomics and Metabolomics Core is dedicated to develop, implement and provide high-quality analytical methodology for determination of molecules involved in disease development, progression and treatment. The standardized techniques offered by Core generate data that can be comparable and cross referenced among specific experiments of individual investigators. Particular attention in the Core is given to quality control. All the methods in the Core are defined by a series of steps to maximize quality of the output and described in Standard Operational Procedures (SOP). Reproducibility is monitored and ensured within the system with procedures implemented to identify any changes and shifts in the platform during sample analyses. This helps to standardize quantitative measurements and facilitate research progress that leads to a better understanding of biological processes.

Services offered by Core Facilities include Sample extraction, High throughput screening, Deep metabolomics and proteomics profiling using LC- and GC-MS techniques, Targeted analysis, Data analysis, Pathway analysis and Flux analysis.

In addition, Molecular imaging is also available. In this case, spatial protein and metabolite distribution is determined using newly established MALDI HiPLEX-IHC technology available through Bruker and AmberGen. It allows for intact protein imaging by combining transcriptome relevant information from labelled protein expression mapping with advanced small molecule imaging from the same tissue section using MALDI Imaging. The method combines MALDI-based mass spectrometry with Immunohistochemistry (IHC) using specific antibodies labeled with a set of photocleavable mass-tags reporters. Platform is used to gain high resolution tissue-wide and single-cell inflammatory RNA and protein expression data with two-dimensional lineage and proximity information.

LCRC 611 (office)

LCRC 627, 928, 941 (labs)

 

CSRB 344 (Office)

CSRB 342 (Metabolomics lab)

CSRB 335 (IT Lab)

CSRB 331 (Proteomics lab)

The Proteomics and Metabolomics Core is well equipped for a variety of experimental samples with instrumentation for liquid and gas chromatography, mass spectrometry, Maldi tissue imaging, Biomarker Analysis, Western Blotting, Multiplex Imaging, and Surface Plasmon Resonance.

Mass Spectrometers and Software

Agilent
Agilent 8890GC coupled to Agilent 7250 GC/Q-TOF
  • Acquisition Rate: 1 to 50 spectra/second independent of m
  • Quadrupole Isolation Mass Range: m/z 20-1050
  • TOF Mass Accuracy: <2ppm for 1 pg OFN at m/z 271.9867
  • TOF Mass Range: m/z 20-3000
  • TOF Mass Resolution (FWHM): > 25,000 at m/z 271.9867 independent of acquisition rate ass resolution

Read more about capabilities of our Agilent 7250 GC/Q-TOF here.

 

Agilent

Agilent 6495 LCQQQ-MS system
  • Mass Range: m/z 5 to 3000
  • Mass Resolution: FWHM around 0.7 Da
  • Scan Modes: Supports MS, MS/MS (product ion, neutral loss/gain, precursor ion), SIM, and MRM (static, dynamic, triggered)

Read more about the capabilities of our Agilent 6495 LCTQ here.

 

Maldi

Maldi Timstof-Flex2 NanoElute System coupled to EvoSep ONE EV1000
  • Dual Source: Designed to switch between ESI and MALDI modes seamlessly for both 4D-Omics and molecular imaging.
  • Laser Source: Smartbeam 3D laser for fast and high-resolution MALDI imaging.
  • Resolution: Up to 60,000.
  • Mass Accuracy: ≤ 2 ppm RMS (external), ≤ 800 ppb RMS (internal).
  • TIMS Analyzer: Separates ions based on their size and shape, improving specificity for molecules in complex samples and tissue sections.
  • Spatial Resolution: Standard at 20 µm, with optional Zoom Mode and microGRID technology for resolutions down to 10 µm or even 5 µm.

Maldi

 

Agilent 1290
Agilent 1290 Infinity II HPLC + Agilent 6475 LC/TQ MS system
  • Mass Range: m/z 5-3000
  • MRM Speed: 500 MRM transitions/s
  • Minimum MRM Dwell Time: 0.5 ms
  • Collision Cell Clearance Time: <1 ms
  • Polarity Switch Speed: <25 ms
  • Quad Resolution (FWHM): Narrow = 0.4 Da, Unit = 0.7 Da, Wide = 1.2 Da, Widest = 2.5 Da
Thermo Fisher
Thermo Fisher Orbitrap Fusion Tribrid mass spectrometer coupled to a Thermo Fisher Dionex Ultimate 3000 nano HPLC system.
  • Resolution: up to 500,000 FWHM at m/z 200
  • Mass Range: Orbitrap detection up to m/z 6000 and linear ion trap detection up to m/z 4000.
  • Scan Rate: up to 20 Hz.
  • Mass Accuracy: <3 ppm RMS using external calibration and <1 ppm RMS with internal calibration.

 
Thermo Fisher Q
Thermo Fisher Q Exactive Plus mass spectrometers coupled to Thermo Fisher Dionex Ultimate 3000 nano HPLC system.
  • Resolving Power: Up to 140,000 at m/z 200 (FWHM)
  • Mass Accuracy: <1 ppm RMS (internal calibration)
  • Mass Range: 50 to 6,000 m/z
  • Scan Speed: Up to 12 Hz
  • Dynamic Range: >5,000:1

 
 
Thermo Proteome Discoverer v2.4 software for qualitative and quantitative mass spec data analysis.

 

 

 

Liquid Chromatography System

Dionex Ultimate
Dionex Ultimate 3000 Multidimensional Liquid Chromatographer with UV/Vis detector
 

Targeted Biomarker Analysis

Olink Flex
Olink Flex

Read more about the Olink Flex’s capabilities here.

 

 

Multiplex Imaging

Lunaphore Comet

Lunaphore Comet
 

Surface Plasmon Resonance

BIAcore 3000
BIAcore 3000 instrument, controller and analysis software
  • Analysis Temperature: 4°C to 40°C.
  • Flow Rate: 1 to 100 µl/min.
  • Sample Handling: Handles up to 192 samples, with automated loading and injection capabilities.
  • MALDI-MS Interface: Enables transfer of interaction partners to MALDI targets for identification.
  • Refractive Index Range: 1.33 - 1.40.

 

 

Sample Preparation Equipment

  • NanoDrop ONE
  • -20 and -80 freezers for sample storage
  • SpeedVac Sample Lyophilyzers
  • Assorted centrifuges and microtube temperature blocks
  • Opentron Flex Robot
  • Labconco Freezone Triad Freeze dryer
  • EVOSEP ONE EV1000

Jiri Adamec, PhD

Professor

Director of Core Laboratories

LSUHSC School of Medicine

Stanley S. Scott Cancer Center

Department of Interdisciplinary Oncology

1700 Tulane Ave, Room 611

New Orleans, LA70112

P: (504) 210-3538

F: (504) 210-2970

E: jadame@lsuhsc.edu