Proteomics & Metabolomics Core
Director
Jiri Adamec, PhD
Director of Core Laboratories
Department of Interdisciplinary Oncology
1700 Tulane Ave, Room 611
New Orleans, LA70112
(504) 210-3538
jadame@lsuhsc.edu
Core Lab Manager
Jennifer Klein
Department of Biochemistry and Molecular Biology
CSRB Lab #331
New Orleans, LA 70112
(504) 568-3152
jklei1@lsuhsc.edu
Location
LCRC 611 (office)
LCRC 627, 928, 941 (labs)
MEB 5312
Mission
The Proteomics and Metabolomics Core supports discovery and innovation by delivering high-quality, standardized analytical approaches that reveal molecular mechanisms of disease and therapeutic response. Our work enables reliable, comparable data that strengthen collaboration and accelerate translational research.
Core Description
The Core Facility provides comprehensive analytical services, including sample extraction, high throughput screening, deep metabolomics and proteomics profiling using LC- and GC-MS techniques, targeted analysis, data processing, pathway analysis and flux analysis.
The central focus of the Core is rigorous quality control. All analytical methods are standardized and documented through detailed Standard Operational Procedures (SOP) designed to maximize data quality and reproducibility. Platform performance is continuously monitored to detect shifts or variability during sample analyses, ensuring consistent quantitative measurements across experiments. These practices support reliable data generation and accelerate research efforts aimed at advancing our understanding of biological processes.
The Core also offers advanced molecular imaging to access spatial protein and metabolite distributions within tissue sections. Using newly established MALDI HiPLEX-IHC technology, intact protein imaging is achieved by integrating antibody-based protein expression mapping with high-resolution small-molecule imaging from the same tissue section. This approach combines MALDI mass spectrometry with immunohistochemistry (IHC), utilizing photocleavable mass-tag–labeled antibodies to enable multiplexed, spatially resolved molecular analysis.
Bruker’s timsTOF flex with ESI and MALDI (LCRC 928)
Fisher Orbitrap Fusion Tribrid (CSRB 331)
ThermoFisher Q Exactive Plus (2) (CSRB 331)
Agilent’s 6495 Triple Quadrupole LC/MS (LCRC 627)
Agilent’s 6475 Triple Quadrupole LC/MS (CSRB 342)
Agilent 7250 GCMS QTOF with EI and CI sources (CSRB 342)
Olink Signature Q100 (LCRC 645)
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|
Service Description |
Metric |
Internal Users |
External Users |
|
Metabolomics |
LC-MRM-MS/MS Targeted Analysis |
each |
$75.00 |
$180.00 |
|
LC-MS Global Profiling - All metabolites (C18) |
each |
$50.00 |
$200.00 |
|
|
LC-MS Global Profiling - Polar metabolites (HILIC) |
each |
$50.00 |
$200.00 |
|
|
LC-MS Lipidomics |
each |
$75.00 |
$200.00 |
|
|
LC-MS/MS Metabolite Identification (Shotgun) |
each |
$60.00 |
$220.00 |
|
|
GC-MS Global Profiling - Polar metabolites |
each |
$50.00 |
$200.00 |
|
|
GC-MS Global Profiling - Fatty Acids |
each |
$50.00 |
$200.00 |
|
|
LC- and GC-MS Combo Profiling |
each |
$70.00 |
$250.00 |
|
|
Instrument Time LC-QQQ-MS |
each |
$30.00 |
$80.00 |
|
|
Instrument Time LC-Orbi-MS |
each |
$40.00 |
$100.00 |
|
|
Instrument Time GC-MS |
each |
$50.00 |
$100.00 |
|
|
Method Development/Sample Preparation (hr) |
each |
$60.00 |
$150.00 |
|
|
Data Processing and Analysis (hr) |
each |
$60.00 |
$150.00 |
|
|
Training (hr) |
each |
$80.00 |
$240.00 |
|
|
Proteomics |
LC-MRM-MS/MS Targeted Analysis |
each |
$120.00 |
$230.00 |
|
LC-MS Profiling |
each |
$65.00 |
$230.00 |
|
|
LC-MS/MS Protein ID (incl in gel/pull down digest) |
each |
$100.00 |
$230.00 |
|
|
Quantification using Shotgun LC-MS/MS |
each |
$140.00 |
$300.00 |
|
|
TMT labeling |
see note |
|||
|
Protein/peptide Label Kit |
each |
$450.00 |
$800.00 |
|
|
Protein/peptide Sample Labeling |
each |
$250.00 |
$400.00 |
|
|
Protein/peptide Labeled Data Processing (hr) |
each |
$200.00 |
$400.00 |
|
|
Instrument Time LC-Qexactive |
each |
$50.00 |
$200.00 |
|
|
Instrument Time LC-Fusion |
each |
$50.00 |
$350.00 |
|
|
Instrument Time LC-timstof-MS |
each |
$50.00 |
$450.00 |
|
|
Method Development/Sample Preparation (hr) |
each |
$140.00 |
$300.00 |
|
|
Data Processing and Analysis (hr) |
each |
$140.00 |
$300.00 |
|
|
Training (hr) |
each |
$200.00 |
$450.00 |
|
|
|
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Notes |
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Typical gradient is 30-60 minutes; if extended separation is needed, there will be extra charge - please talk to PMC representative for details |
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For a method development and implementation, extra charges can be applied to purchase methods related to chemicals, standards, columns or other necessary items - please talk to PMC representative for details and the initial (setup) cost. Depending on method complexity, it usually takes 4-8 weeks to validate the method. |
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- Untargeted proteomics/metabolomics/lipidomics
- Targeted proteomics/metabolomics/lipidomics
- Label Free Quantification (relative)
- Isotope-based Tag Quantification (relative and absolute)
- Molecular Identification (proteins/metabolites/lipids)
- PTMs