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Keywords Associated with Work
Amputation
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Arthrofibrosis
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Blastema
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Fibroblasts
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Fibrosis
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Healthcare Disparities
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Hyperbaric Oxygenation
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Inflammation
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Joint Replacement
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Limb Trauma
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Matrix Remodeling
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Osteoarthritis
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Regeneration
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Scaffold
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Wound Healing
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Primary Research / Clinical / Teaching Interests
Anti-inflammatory and anti-fibrotic therapy for arthritis
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Assessment of osteoarthritis-attributable myopenia
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Cannabinoid-based management of pain and inflammation in arthritis
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Histopathology of non-neoplastic and cancer lesions
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Repair and regeneration in limb trauma and ischemia reperfusion injury
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Secondary Research / Clinical / Teaching Interests
Cancer biology and prognosis
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Energy metabolism in tissue salvage
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Unique Equipment Used
Bio-Rad Radiance 2100 Laser Scanning Confocal System
Confocal optical sectioning of thick biological samples labeled with fluorescent reporter molecules and probes for subsequent three-dimensional rendering and analysis. CSRB 532 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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Confocal Microscope
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Cryotome
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Epifluorescent Microscope
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Fluoview FV1000 multiphoton system
Laser scanning microscope collects 2D, 3D, and timelapse images through pulsed, infrared laser excitation that allows high resolution fluorescence imaging deep within specimens without compromising viability CSRB 530 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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In Vivo Biophotonic Imager
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Kodak In Vivo Image Station FX
Provides x-ray and high performance optical molecular imaging of near-infrared, fluorescent, radioisotopic, and luminescent reporters in live, small animals. CSRB 530 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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Laser Capture Microdissection Scope
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Leica CM3050S cryostat
collects thin sections of frozen specimens for histology CSRB 532 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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Leica DMRXA deconvolution microscopy systems (2X)
Allow the collection of light diffraction-corrected 2D, 3D, and timelapse photomicrographs involving the detection, localization, motility, interaction, and quantitation of fluorescent molecules tagged to cells and tissues. CSRB 532 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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Leica Mz74 stereoscope
basic transmitted and epifluorescence imaging of gross specimens CSRB 532 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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Light Microscope
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Micro-CT
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Mutiphoton Microscope
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Nikon E600 upright microscope
basic transmitted and epifluorescence imaging of cell and tissue samples CSRB 530 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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PALM Microbeam
Laser microdissection instrument enables non-contact dissection of areas within a tissue or cell culture specimen into microcentrifuge tubes for subsequent DNA and/or RNA analysis. CSRB 532 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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Perkin Elmer GX2 micro-CT scanner
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Shandon Finesse Paraffin Microtome
collects thin sections of paraffin-embedded samples for histology CSRB 532 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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Shandon Histocentre Paraffin Embedding Station
houses tissue samples within a paraffin mold for subsequent histological sectioning CSRB 532 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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Shandon Hypercenter XP Paraffin Processor
automated fixation, dehydration, clearing, and paraffin infiltration of tissue samples for subsequent histology CSRB 532 - MIC Contact Person: Dr.Luis Marrero RECHARGE
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Shandon Varistain 24-4
automated slide stainer for routine, special stain, and immunohistochemical slide pretreatment for histopathological analysis CSRB 532 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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Xenogen IVIS 200
Utilizes in vivo biophtonic imaging to non-invasively image, record, and quantitate cellular and genetic activity within an anesthetized organism. CSRB 530 - MIC Contact Person: Dr. Luis Marrero RECHARGE
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X-ray Imager
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Unique Techniques Used or Developed
ATP in vivo electrophysiology, in vivo bioluminescence, laser capture microdissection, limb amputation and joint excision surgery, immunohistochemistry, immunohistofluorescence, immunocytofluorescence, whole mount fluorescence, biophotonic imaging in vivo, hyperbaric therapy, live cell imaging, resonance energy transfer, fluorescence recovery after photobleaching, histopathology scoring, RT-PCR, multiplex assays, ELISA, stereomicroscopy, confocal microscopy, multiphoton microscopy, deconvolution microscopy
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ATP in vivo electrophysiology, in vivo bioluminescence, laser capture microdissection, limb amputation and joint excision surgery, immunohistochemistry, immunohistofluorescence, immunocytofluorescence, whole mount fluorescence, biophotonic imaging in vivo, hyperbaric therapy, live cell imaging, resonance energy transfer, fluorescence recovery after photobleaching, histopathology scoring, RT-PCR, multiplex assays, ELISA, stereomicroscopy, confocal microscopy, multiphoton microscopy, deconvolution microscopy
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ATP In Vivo Electrophysiology
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Biophotonic Imaging In Vivo
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Deconvolution Microscopy
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Enzyme-Linked Immunosorbent Assay
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Fluorescence Recovery After Photobleaching
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Fluorescence Resonance Energy Transfer
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Histopathology Scoring
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Hyperbaric Oxygenation
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Immunohistochemistry
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immunohistofluorescence
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In Vivo Bioluminescence
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Laser Capture Microdissection
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Limb Amputation and Joint Excision Surgery
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Live Cell Imaging
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Microscopy, Confocal
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Microscopy, Fluorescence, Multiphoton
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Multiplex Assays
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Reverse Transcriptase Polymerase Chain Reaction
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Stereomicroscopy
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Whole Mount Fluorescence
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