Macrophage Chemotactic Factor
- When lymphocytes that are sensitized to a specific antigen
(in this case histoplasmin) are incubated with that antigen, they produce a
soluble macrophage chemotactic facotr. This factor diffuses away from
the lymphocytes, forming a concentration gradient which directs the
macrophages to the site.
- Look at the photo. Three wells were cut in an
agar covered slide. Normal macrophages were placed in the center well.
- Histoplasmin sensitized lymphocytes were mixed with histoplasmin. The
liquid (no cells) from this mixture was placed in the right well.
- Normal
(unsensitized) lymphocytes were mixed with histoplasmin. The liquid (no
cells) from this mixture was placed in the left well.
- Migration of
macrophages outward from the center well was accelerated only in
the direction of the gradient of MCF originating from the top well.
- The
chemostatic index is the ratio of distance A to distance B. In the example
shown, the index is 2.0.
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Comments to MIPmaster: hthomp@lsumc.edu
Revised: October 7, 1997
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